PROTOPLAST FORMATION AND MANDELIC ACID DEHYDROGENASE LOCALIZATION INPSEUDOMONAS FLUORESCENS
نویسندگان
چکیده
منابع مشابه
Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase
OBJECTIVES (S)-Mandelate dehydrogenase (SMDH) and laccase were immobilized on chitosan. The bi-enzymatic system with immobilized SMDH and immobilized laccase was taken to catalyze the stereoselective transformation of racemic mandelic acid and (R)-mandelic acid was obtained from its racemic mixture. RESULTS Characteristics of the immobilized enzymes were valuated. The optimum pH and temperatu...
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Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...
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Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/ mL), in an osmotic solution for 90 min at 28oC. The frequency of regenerated pro...
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An enzymatic method is described for the measurement of vanillyl mandelic acid (VMA). The method utilizes an L-mandelic acid dehydrogenase preparation from Pseudomonas fluorescens A-312 to catalyze the transformation of VMA to vanillin. The method is specific for VMA becauseonly the final product, vanillin, will absorb maximally at 350 m/L. The results compare favorably with the method of Pisan...
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ژورنال
عنوان ژورنال: Japanese Journal of Microbiology
سال: 1958
ISSN: 0021-5139
DOI: 10.1111/j.1348-0421.1958.tb00063.x